The Roosevelt Train Station a secret subterranean extension of New York's D-line train under Pershing Square seen in The Amazing Spider-Man 2. It is the location of Richard Parker's secret laboratory.
The station was originally built in the 1940s to transport President Franklin Roosevelt away from the public eye to keep his Polio hidden from the American people. Richard Parker took the D-line train to work every day and at some point discovered the Roosevelt train station and built a secret lab in an abandoned subway car. Years later Richard's son Peter Parker discovered secret subway tokens in his father's calculator which led to him discovering the location of the lab and gaining entry. Peter discovered a message from his father in the lab which detailed his father's work.